9 the tox cuvette test (lck488) – Hach-Lange LUMISTOX 300 LPV321 User Manual User Manual
Page 22

LUMIStox 300 Operating Manual
Dr. Bruno Lange GmbH
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Page: 22
2.9 The TOX cuvette test (LCK488)
The TOX cuvette test is a luminescent bacteria test for operational analysis. One
package of preserved luminescent bacteria enables one sample to be determined.
Work steps:
1.
Thaw blank solution (labelled “NULL”).
2.
Prepare sample.
3.
Reactivate preserved bacteria as described in package circular and add 0.4 ml to
the zero cuvette and the sample cuvette.
4.
Select the evaluation mode <Test> and the TOX cuvette test <488> for LCK488
on the LUMIStox 300.
5.
Measure the initial luminescence of the zero cuvette and the sample cuvette.
6.
Quickly add 0.4 ml zero-solution or sample to the bacteria suspensions.
7.
Wait for the incubation period of 15 minutes to elapse.
8.
Measure the final luminescence of the “NULL” cuvette and sample cuvette.
9.
Record or print the results.