Hach-Lange LUMISTOX 300 LPV321 User Manual - Manual (Page 24)

LUMIStox 300 Operating Manual Dr. Bruno Lange GmbH
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Page: 24
2.10 Determining optical density (OD)
The LUMIStox 300 measuring instrument incorporates a photometer function for
determining the extinction of a sample as optical density (OD) at a wavelength of 485
nm. The photometer function checks whether preliminary dilution of a strongly coloured
sample is necessary, because the LUMIStox 300 can only carry out colour correction
up to an extinction of 1800 mE. It is also used to assess bacterial growth in the
luminescent bacteria test for chronic toxicity, LUMIS·24·tox (LCK486).
LUMIStox measuring cuvettes (LZP187) are used to carry out the measurements.
Work steps:
1. Add at least 1 ml water, 2% NaCl solution or any other blank solution to the blank
cuvette.
2. Add at least 1 ml sample to the sample cuvettes.
Select program mode <OD> in the 2nd level
of the main menu.
Perform blank balancing of LUMIStox 300 with blank
cuvette.
Measure sample.
Record or print result.
t e s t O D c h r o n i
r e fe r e n c e c h e c k
p l e a s e w a it
b l a n k - c u v e tt e
b l a n k
s a m p le
m e a s u r e
0 .9 1 E
b l a n k