Leica Biosystems CM1850 UV - Manual (Page 26)

26 Instructions for use V 1.5 – 10/2012
7. Daily use of the instrument
7.1 Specimen freezing
• Select the appropriate sectioning temperature (cryochamber tempera-
ture) for the sample material (please refer to 7.4 Temperature selection
chart‘).
7.1.1 Quick freeze shelf
The cryochamber has a quick freeze shelf (5, Fig. 17) for up to 10 specimen discs.
The temperature of the quick freeze shelf is always lower than the cryochamber
temperature.
• Cut the specimen to size.
• Activate the Peltier element (4), if available - it
may take up to 40 seconds until the maximum
refrigeration output is available.
• Apply enough cryocompound to a specimen
disc at room temperature.
• Place the specimen on the disc and orient.
• Place the specimen disc in one of the holes of
the quick freeze shelf and freeze the speci-
men at a low temperature.
• Once the specimen is frozen, insert the speci-
men disc in the specimen head
(Fig. 18) and start sectioning.
7.1.2 Stationary heat extractor
• Fix the support (1) of the heat extractor (2) by tightening the 2 screws to
the threaded holes on the left sidewall of the cryochamber and insert the
heat extractor.
• Lower the heat extractor cylinder on the specimen surface. After ap-
proximately 30 seconds contact, the specimen will be entirely frozen.
• Once the specimen is frozen, place the heat extractor on the parking
station (3).
1
2
3
4
5
Fig. 17