Leica Biosystems CM1850 UV - Manual (Page 40)

40 Instructions for use V 1.5 – 10/2012
9.2 Spray disinfection with Leica Cryofect
Basically, we recommend UV disinfection (see page 21 for detailed instruc-
tions).
For easy-to-use spray disinfection we recommend Leica Cryofect.
(Cryofect is not available in all countries!)
The cryostat has to be disinfected after each daily use.
Comply with the instructions for use!
The glass anti-roll plate can remain in place during disinfection.
1. Select a cryochamber temperature value down to -20 °C.
2. Remove the knife or blade from the knife holder.
3. Remove all samples, microscope slides and tools from the cryochamber.
4. Remove debris from the cryochamber.
Allow the cryochamber to reach the previously selected temperature.
Once the selected temperature is reached, either
5a. spray the disinfectant evenly on the contaminated surfaces- the sur-
faces should be covered with an even layer - or
5b. soak a cloth with disinfectant and apply it on the contaminated surfaces.
6. Allow a reaction time of no less than 15 minutes.
7. Wipe it off with a tissue.
8. Dispose of tissue in compliance with the ruling waste disposal regula-
tions of your institution.
9. Set the cryochamber temperature to the originally selected value.
If increased frost buildup occurs, start a manual defrost cycle.
9. Cleaning, disinfection, maintenance