Leica Biosystems CM1850 UV - Manual (Page 34)

34 Instructions for use V 1.5 – 10/2012
8. Troubleshooting
8.3 Possible causes and remedies
Frost on chamber walls and mi-
crotome
Ice formation on the bottom of
the cryochamber
Sections smear
Sections splinter
Sections not properly flattened
Problem Cause Remedy
- Cryostat is exposed to air cur-
rents (open windows and
doors, air conditioning).
- Sliding window was open and
exposed to air currents too
long.
- Frost built up by breathing into
the cryochamber.
- Condensation water drain ob-
structed.
- Drain of the quick freeze shelf
defrosting water obstructed.
- Specimen not cold enough.
- Knife/blade and/or anti-roll
plate not yet cold enough and
thus warm the sections.
- Specimen too cold
- Static electricity/air currents.
- Specimen not cold enough.
- Large area specimen.
- Anti-roll plate poorly posi-
tioned.
- Anti-roll plate poorly aligned
with knife edge.
- Incorrect clearance angle.
- Knife/blade blunt or damaged.
- Change place of installation for
the cryostat.
- Open the tap of the drain tube
(2, Fig. 31), switch off the instru-
ment and let it thaw and dry.
- Align the instrument with a
spirit level.
- Select lower temperature.
- Wait until knife/blade and/or
anti-roll plate have reached
chamber temperature.
- Select higher temperature.
- Remove cause.
- Select lower temperature.
- Trim the specimen parallel,
increase section thickness.
- Reposition anti-roll plate.
- Align correctly.
- Set correct angle.
- Use different part of the cutting
edge or replace.