Leica Biosystems CM1850 UV - Manual (Page 35)

35 Leica CM1850 UV – Cryostat
Problem Cause Remedy
Sections not properly flattened
despite correct temperature and
correctly aligned anti-roll plate
Sections curl on the anti-roll
plate
Scraping noise during sectioning
and specimen return movement
Ridged sections
Chatter during sectioning
- Knife/blade and/or anti-roll
plate dirty.
- Edge of anti-roll pate damaged.
- Blunt knife/blade.
- Anti-roll plate does not pro-
trude far enough beyond the
cutting edge.
- Anti-roll plate protrudes too far
beyond the cutting edge and is
scraping against the specimen.
- Knife/blade damaged.
- Edge of anti-roll plate dam-
aged.
- Specimen insufficiently frozen
onto the specimen disc.
- Specimen disc not clamped
tightly.
- Specimen holder ball joint not
clamped.
- Knife/blade not clamped tightly
enough.
- Specimen has been sectioned
too thickly and has detached
from the disc.
- Very hard, inhomogeneous
specimen.
- Blunt knife/blade.
- Clean with dry cloth or brush.
- Replace plate.
- Use different part of the cutting
edge or replace.
- Readjust correctly.
- Readjust correctly.
- Use different part of the cutting
edge or replace.
- Replace the plate.
- Refreeze specimen onto the
disc.
- Check disc clamping.
- Check ball joint clamping.
- Check knife/blade clamping.
- Refreeze specimen onto the
disc.
- Increase section thickness;
reduce specimen surface area
if necessary.
- Use different part of the cutting
edge or replace the knife/
blade.
8. Troubleshooting