5
Leica CM1850 UV – Cryostat
Table of contents
7.
Daily use of the instrument ................................................................................................................. 26
7.1
Specimen freezing ............................................................................................................................................... 26
7.1.1
Quick freeze shelf ................................................................................................................................................ 26
7.1.2
Stationary heat extractor ................................................................................................................................... 26
7.2
Specimen discs ................................................................................................................................................... 27
7.2.1
Inserting the specimen discs in the specimen head ..................................................................................... 27
7.2.2
Specimen orientation .......................................................................................................................................... 27
7.3
Sectioning ............................................................................................................................................................. 28
7.3.1
Trimming the specimen ...................................................................................................................................... 28
7.3.2
Section thickness setting ................................................................................................................................... 28
7.4
Temperature selection chart (in minus °C) .................................................................................................... 29
7.5
Defrosting ............................................................................................................................................................. 30
7.5.1
Automatic defrosting of the cryochamber ...................................................................................................... 30
7.5.2
Manual defrosting of the cryochamber ........................................................................................................... 30
7.5.3
Manual defrosting of the quick freeze shelf ................................................................................................... 31
7.6
Terminating work ................................................................................................................................................. 31
7.6.1
Terminating daily work ....................................................................................................................................... 31
7.6.2
Shutdown for a longer period ............................................................................................................................ 32
8.
Troubleshooting .................................................................................................................................. 33
8.1
Error messages in the display ....................................................................................................................... 33
8.2
Temperature control button .......................................................................................................................... 33
8.3
Possible causes and remedies ..................................................................................................................... 34
9.
Cleaning, disinfection, maintenance ............................................................................................ 39
9.1
Cleaning ............................................................................................................................................................... 39
9.2
Spray disinfection with Leica Cryofect ...................................................................................................... 40
9.3
Maintenance ...................................................................................................................................................... 41
9.3.1
General maintenance ...................................................................................................................................... 41
9.3.2
Removal of the microtome .............................................................................................................................. 42
9.3.3
Removal of the microtome cover .................................................................................................................. 44
9.3.4
Reinstallation of the microtome ................................................................................................................... 44
9.3.6
Replacement of UVC lamp .............................................................................................................................. 46
9.3.5
Replacement of the fuses .............................................................................................................................. 46
9.3.7
Replacement of the lamp ............................................................................................................................... 48
10.
Ordering information, optional accessories ............................................................................... 49
10.1
Ordering information ........................................................................................................................................ 49
10.2
Optional accessories ....................................................................................................................................... 51
10.2.1 Mobile heat extractor ...................................................................................................................................... 5110.2.2 Thermal block ..................................................................................................................................................... 51
11.
Warranty and service ........................................................................................................................ 52
12.
Decontamination Certificate (master) ........................................................................................... 53
13.
Information for the People´s Republik of China .......................................................................... 55
