Leica Biosystems CM1850 UV - Manual (Page 28)

28 Instructions for use V 1.5 – 10/2012
7.3 Sectioning
All necessary adjustments on the knife holder and anti-roll guide
are described in the separate instruction manual for your knife
holder in detail.
7.3.1 Trimming the specimen
Take care when handling microtome knives and disposable
blades. The cutting edge is extremely sharp and can cause severe
injury!
• Insert the precooled knife/blade in the knife holder and clamp.
• Adjust the appropriate clearance angle on the knife holder. Adjustments between 4 ° and 6 ° (knife
holder CN and CE-TC) or between 1° and 2 ° (knife holder CE) are suitable for most applications.
• Align the knife holder/knife with the specimen.
• Remove the knife guard (knife holder CN) or fold the glass anti-roll guide (knife holder CE, CE-TC)
over to the left.
• Unlock the handwheel.
• Trim the specimen to shape approach the specimen towards the knife by means of the coarse feed
buttons. Trim the specimen down to the required sectioning plane by turning the handwheel.
• Position the anti-roll guide on the knife and align with the cutting edge.
Readjust the anti-roll plate if necessary.
7.3.2 Section thickness setting
The section thickness is adjusted in a range of 1 to 60 μm by turning the knob (1) :
from 0 - 10 μm in 1 μm increments,
from 10 - 20 μm in 2 μm increments,
from 20 - 60 μm in 5 μm increments.
The selected section thickness is indicated on the index mark on the micro-
tome.
•
Select the required section thickness with the control knob on the
microtome.Start sectioning at approximately 20 μm.
• Decrease the section thickness continually down to the appropriate value.
After changing from one section thickness to another, the first two or three sections should be
rejected.
• When sectioning, turn the handwheel at a constant speed.
7. Daily use of the instrument
Fig. 19