Leica Biosystems ANGLE TWO - Manual (Page 43)

Using the Instrument
Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 43
Preparation
1. Ensure that the instrument is clean (see “Cleaning and Maintenance” on page 47).
2. Turn the computer on and start the Angle Two software (see Figure 3).
3. Align the instrument using the alignment kit (see “Alignment” on page 33).
Selecting the Target Site
The software opens in Set Target mode.
4. Enter the target site into the Target (Red) boxes beneath using one of the following options
(see “Set Target” on page 26):
(i) Select the site on the atlas
(ii) Open saved coordinates
(iii) Manually type in the coordinates
5. If using offsets (see “Initial Settings” on page 29) click Setup and check the Use Zero
Offsets box (see Figure 45), and enter the values.
6. Unlock the rotation and tilt clamps and move the manipulator to the angle you want to use to
approach the target site. Relock the clamps when positioned.
Positioning the Animal
If repeat surgery is planned the position of the animal must be easily reproducible. Always use ear
bars and an incisor bar suitable for the animal.
Installing the Ear Bars
7. Set the left ear bar tip 1 cm left of the center position, and lock it in place.
8. Remove the right ear bar and leave the lock ‘open’.
Figure 48. The left ear bar locked 1 cm from the center position and the
right ear bar removed with the lock ‘open’
9. Unscrew the incisor bar’s horizontal screw (see Figure 5) and slide the incisor bar to the rear of
the instrument.
10. Hold the animal’s body with one hand and the head with the other hand. Move the animal so
that the left ear bar is inside the ear canal. Point the animal’s nose slightly to the right to help
with insertion.
Locked Unlocked