Leica Biosystems ANGLE TWO - Manual (Page 46)

Using the Instrument
Leica Angle Two ™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 46
Probe Placement
30. Unlock the swing-out clamp and reposition the manipulator and probe over the animal.
31. Relock the swing-out clamp.
32. Use the coarse DV drive to approach the target site.
33. Engage the fine drive when about to enter the brain.
A single blue circle appears around the blue crosshairs when the probe is 0.05 mm from the
target site (see Figure 50).
34. Stop moving the probe once a second blue circle appears around the blue crosshairs. This
indicates that the target site has been reached.
35. Perform the operation, and when complete withdraw the probe using the fine drive until the
probe is clear of the brain.
If you are performing bilateral surgery:
(i) Select Set Target mode and click +/- (see Figure 25), to change the sign of the ML
coordinate.
(ii) Move the probe to the target site and repeat steps 22 to 32.
36. Remove the animal from the instrument and close up the incision.
Figure 50. A single blue circle appears when the probe is 0.05 mm from the target.
A second blue circle appears when the probe is at the target site.