LaMotte SMART Spectro Spectrophotometer - Manual (Page 189)

PROCEDURE
Use universal sample holder.
1. Use the 10 mL graduated cylinder (0416) to measure 10 mL of sample water.
Pour into glass test tube (0608).
2. Use the 1 mL pipet (0354) to add 1 mL of *Hydrochloric Acid, 2.5N (6251).
3. Use the 0.1 g spoon (0699) to add 2 measures of *Ammonium Persulfate
Reagent (6566). Add two drops of *Silver Nitrate Solution, 0.0141N (6346WT).
Mix until the powder has dissolved. The solution will be slightly cloudy at this
point.
4. Use 10 mL graduated cylinder (0416) to add 5 mL of Sodium Citrate, 10%
(6253).
5. Use a second 1 mL pipet (0354) to add 1 mL of *Ammonium Hydroxide, Conc.
(6537). Mix, then dilute to 25 mL with deionized water.
6. Use a third 1 mL pipet (0354) to add 1 mL of *Dimethylglyoxime, 1% (6254).
Mix. Wait 20 minutes for color development.
7. At end of 20 minute waiting period fi ll a clean tube (0290) to the 10 mL line with
the developed test sample.
8. Fill a second clean tube (0290) to 10 mL line with deionized water or untreated
sample water. This is the blank.
9. Press and hold ON button until spectrophotometer turns on.
10. Scroll to and select PROGRAMMED TESTS.
11. Scroll to and select ALL TESTS (or another sequence containing
63 Nickel) from TESTING MENU.
12. Scroll to and select 63 Nickel from menu.
13. Insert the blank into chamber, close lid and select SCAN BLANK.
14. Insert test sample into chamber, close lid and select SCAN SAMPLE. Record
result.
15. Press OFF button to turn spectrophotometer off or press EXIT button to exit to
a previous menu or make another menu selection.
NICKEL SMART Spectro Test Procedures 2.11
Test P
rocedures