LaMotte SMART Spectro Spectrophotometer - Manual (Page 228)

PROCEDURE
Use universal sample holder
1. Preheat COD reactor to 150 ±2°C. Follow safety precautions.
2. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use a 1.0
mL pipet (0354) to add 5.0 mL of sample.
3. Use the 0.15 g spoon (0727) and a funnel (0459) to add one level measure of
*Digestion Reagent Powder (4036). Tap funnel to dispense powder completely.
Cap tube tightly and shake until powder dissolves completely.
4. Place the tube in the COD reactor for 30 minutes.
5. At the end of the heating period, turn the reactor off. Carefully remove the tube
from the reactor and allow it to cool to room temperature.
6. At the end of the cooling period, press ON button until spectrophotometer
turns on.
7. Scroll to and select PROGRAMMED TESTS from menu.
8. Scroll to and select ALL TESTS (or another sequence containing
82 Phosphorus T LR from PROGRAMMED TESTS menu.
9. Scroll to and select 82 Phosphorus T LR from the menu.
10. Carefully remove the caps from the digested tube. Use another 1 mL pipet
(0354) to add 1.0 mL of *Total Phosphorus LR Hydroxide Reagent (4038) to the
tube. Cap and invert to mix.
11. Wipe the vial with a damp towel to remove fi ngerprints and smudges. Wipe
with a dry towel.
12. Insert the tube into the chamber. Select SCAN BLANK. Remove the tube from
the spectrophotometer.
13. Use another 1 mL pipet (0354) to add *1.0 mL of Phosphate Acid Reagent (V-
6282). Cap and invert tube to mix.
14. Use the 0.1g spoon (0699) and a funnel (0459) to add one level spoon of
Phosphate Reducing Reagent (V-6283). Tap funnel to dispense powder
completely. Cap tube and shake until powder dissolves.
15. Wait 5 minutes.
16. Wipe the tubes with a damp towel to remove fi ngerprints and smudges. Wipe
with a dry towel.
17. Insert the tube into the chamber. Select SCAN SAMPLE. Record the result as
Total Phosphorus in mg/L PO
4
.
18. Press OFF button to turn the spectrophotometer off or press EXIT button to
exit to a previous menu or make another menu selection.
NOTE: For greater accuracy, use laboratory grade pipets.
SMART Spectro Test Procedures 2.11 PHOSPHORUS-TOTAL, Low Range
Test P
rocedures