Bio-Rad EXQuest Spot Cutter User Manual

Page 10

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Gel Tip Wash Station. The gel tip wash station is located on the left end of the wash station block. It
consists of a gel tip wash port, a waste basin, and a waste level sensor. The gel tip is cleaned as it
is positioned in the wash port and water is pumped through the cutting tip and up around the tip's
outer surface and then down to the waste basin. The waste water level sensor detects when the
basin is full and causes its contents to be pumped to the waste reservoir.

Membrane Tip Wash Station. The membrane tip wash station consists of three wash ports in which
three 1.5 ml capless tubes (catalog #165-7212) are placed. These tubes need to be filled with water
(or an appropriate wash solution) to within 1 to 2 mm of the tube top before they are placed in the
membrane tip wash ports. During a cut run, the tip is dipped in one of the three tubes after each cut
to both rinse the membrane tip's outer surface and ensure that any attached membrane debris is
removed from the cutting tip.

1.2.7

Microplate Rack

Figure 1-7. Microplate rack.

The microplate rack is a two-position reversable rack located to the left of the gel tray. It holds microplates
that have a foot print compatible with the SBS standard for microplates. When the rack is positioned as
shown in the figure above, it accommodates standard height microplates (14 mm high). When inverted, it
accommodates deep-well microplates (41 to 44 mm high) and microtube racks (41 to 48 mm high). The
microplate rack holds up to four 96-tube racks, 96-well plates or 384-well plates.

Closely

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Widely

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INTRODUCTION

1-6

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