Bio-Rad EXQuest Spot Cutter - Manual (Page 2)

TABLE OF CONTENTS
TABLE OF CONTENTS
SAFETY
SECTION 1 INTRODUCTION ..................................................................................................................1-1
1.1 Overview................................................................................................................................1-1
1.2 System Description................................................................................................................1-2
1.2.1 Gel Imaging System ................................................................................................1-2
1.2.2 Imaging System ........................................................................................................1-3
1.2.3 Gel Tray and Gel Clamps..........................................................................................1-3
1.2.4 Cutting Heads ..........................................................................................................1-4
1.2.5 Fluidics System ........................................................................................................1-5
1.2.6 Wash Solution ..........................................................................................................1-5
1.2.7 Microplate Rack ........................................................................................................1-6
1.2.8 Robotics....................................................................................................................1-7
1.2.9 Sensors ....................................................................................................................1-8
1.2.10 Front Panel Controls and Indicators ........................................................................1-8
1.2.11 Software....................................................................................................................1-9
1.2.12 Barcode Reader........................................................................................................1-9
SECTION 2 SYSTEM INSTALLATION ....................................................................................................2-1
2.1 Pre-Installation ......................................................................................................................2-1
2.2 Unpacking the EXQuest Spot Cutter ....................................................................................2-1
2.3 System Leveling ....................................................................................................................2-3
2.4 Gel Tray Installation ..............................................................................................................2-3
2.5 Microplate Rack Installation ..................................................................................................2-4
2.6 Cable Setup ..........................................................................................................................2-5
2.7 Cutting Head Installation ......................................................................................................2-6
2.7.1 Gel Cutting Head ......................................................................................................2-6
2.7.2 Membrane Cutting Head ..........................................................................................2-7
2.8 DI Water and Waste Bottle Installation ..................................................................................2-8
2.9 Software Installation ..............................................................................................................2-8
2.10 Starting the Software ............................................................................................................2-9
SECTION 3 EXQUEST CALIBRATION WIZARD ....................................................................................3-1
SECTION 4 GEL AND BLOT PREPARATION ........................................................................................4-1
4.1 Free Gels ..............................................................................................................................4-1
4.2 Plastic and Glass-Backed Gels ............................................................................................4-2
4.3 Blot Preparation ....................................................................................................................4-2
SECTION 5 MANUAL EXCISION TOOL. ................................................................................................5-1
5.1 Performing a Manual Excision Tool Cut Run ........................................................................5-1
5.2 Importing a Cut List ..............................................................................................................5-4
5.3 Confirm Cuts..........................................................................................................................5-5
5.3.1 Identifying Re-cuts From a Microplate Image ..........................................................5-5
5.3.2 Identifying Re-cuts From a Gel Image......................................................................5-7
SECTION 6 ANALYSIS SET EXCISION TOOL ......................................................................................6-1
6.1 Setting Up An Analysis Set Excision Run ............................................................................6-1
6.2 Starting A Cut Run ................................................................................................................6-6
6.3 Analysis Set Excision Tool – Advanced User Mode ............................................................6-13
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