Bio-Rad EXQuest Spot Cutter User Manual

Page 7

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INTRODUCTION

1-3

Figure 1-3. Light enclosure.

1.2.2

Imaging System

The gel and blot imaging system consists of a CCD camera (1600 x 1200 pixels, 12 bit ADC, pixel size
230 microns) fitted with a 620 (Center line) /60 (width) nm band pass filter that is optimized for SYPRO Ruby
protein gel stain. It also encompasses two fluorescent UV EPI lights, located above the gel tray; and a Trans
white light source, located under the gel tray

1.2.3

Gel Tray and Gel Clamps

The gel tray securely holds gels and membranes to be imaged and cut. Its features include:

Large surface area: The gel tray’s large size (32 x 28 cm) allows it to hold one large gel or up to four
small gels (e.g., Criterion™ gels) without the need for trimming.

Gel placement guides: The gel tray is marked to help position gel(s) and blots. Gels and blots should
be positioned near the center of the gel tray for best performance.

Removable design: The gel tray can be easily removed from the spot cutter so that gels and blots
can be placed on it from the convenience of the lab bench.

Gel Clamps: The gel tray includes clamps used to hold gels and blots securely in place.

Protective Sheets: The disposable gel cutting sheets (patent pending)are used to protect the gel tray
surface from damage by the cutting tips (see Accessories Section 9 for ordering information). Failure
to use the protective cutting sheets will damage the gel tray and void its warranty. Cutting sheets can
also be used to lift gels out of storage trays to minimize damage from excessive handling.

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