Bio-Rad EXQuest Spot Cutter User Manual
Page 35
4.3 BLOT
PREPARATION
1.
Store PVDF and nitrocellulose blots in a container that keeps them protected and hydrated until
needed for spot cutting.
2.
Remove the gel tray from the spot cutter and place a cutting sheet on it. A cutting sheet should
always be used to cover and protect the gel tray from being scored by the cutting tip. Remove the
thin plastic film from the cutting sheet before using it.
3.
If a single blot is being cut, position it in the middle of the gel tray (see Figure 4-1A). If two to four
small blots are being cut using the Analysis Set Excision Tool, position them as indicated on the
screen (see Figure 4-1B). Avoid excess water on the cutting sheet as this may cause the blot to float
and decrease the spot cutting performance.
4.
Position the gel clamps on the blot.
5.
Insert the gel tray into the spot cutter and make sure it is latched securely by pushing it toward the
rear until it snaps into place.
GEL AND BLOT PREPARATION
4-2