Bio-Rad EXQuest Spot Cutter - Manual (Page 50)

Figure 6-10. Place Gel/Blot on Spot Cutter dialog box: Single Gel Placement
Figure 6-11. Place Gel/Blot on Spot Cutter dialog box: Multiple Gel Placement
9. Place the gel(s) or blot(s) on the gel tray and position them as shown in the image (see Figures 6-10
and 6-11 for an example). Make sure the gels or blots are held securely in place by the clamps
provided with the spot cutter (see Section 4.1). Failure to clamp the gel or blot may result in
decreased accuracy and low cut yields.
10. Select the light source. Use White Trans for visible stains, such as Coomassie or silver stain, or UV
EPI for fluorescent stains, such as SYPRO Ruby.
11. Click Acquire image to image the cutting deck.
12. Toggle between the Analysis image(s) showing placement and Image of cutter stage images to
ensure that the gels have been placed on the platform in the correct location and orientation.
13. Click Proceed to continue to the "Align Analysis and Cutter Images" dialog box, or Cancel to return
to the "Analysis Set Excision Control Tool" window.
ANALYSIS SET EXCISION TOOL
6-8