Bio-Rad EXQuest Spot Cutter - Manual (Page 28)

EXQUEST CALIBRATION WIZARD
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8. Lens Calibration. The lens calibration step corrects for image distortion due to the camera lens. To
perform the lens calibration, place the Calibration Target on the gel tray and align it with the front left
corner of the gel tray. Click Next to perform the lens calibration, Back to repeat the previous step or
Cancel to abort the calibration.
Figure 3-8. “Lens Calibration” screen.
9. System Position Calibration. The system position calibration step aligns the image coordinates with
those of the robot. Remove the calibration target from the gel tray and place 9 calibration pucks in a
3 x 3 evenly spaced grid on the gel tray as shown in Figure 3-9. Perform an automatic or manual
calibration. Note that automatic and manual calibration will give identical results. However, automatic
calibration requires no user interaction. Automatic calibration is only available if the spot cutter has
been previously calibrated.