Bio-Rad EXQuest Spot Cutter - Manual (Page 69)

EXCISION TOOL CONTROLS
8-5
• Define hydration area. To define the cutting deck area to hydrate, click this button and then click and
drag a box around the gel or blot. This function is not present in the Analysis Set Excision Tool since
the gel location is identified by the position of the green data area box in the “Align Analysis and
Cutter Images” window.
• Hydrate Now. Click this button to immediately hydrate the gel. Note that if Hydrate is checked, the
gel/blot will be hydrated before the run starts.
8.7 WASH OPTIONS PANEL
Figure 8-7. “Wash Options” panel
The Wash Options panel is used to set the water volume required to wash the gel tip. This tab is not
available in membrane cutting mode.
8.8 RUN INFO PANEL
Figure 8-8. “Run Info” panel
The Run Info panel provides information about the cut run as it is set up.
• Cuts: This field displays the number of cuts requested and includes multiple cuts made from the
same spot.
• Wells: This field displays the number of microplate wells required for the cut run. This number will
always be less than or equal to Cuts since multiple cuts made from one spot always go into the
same well.
• Plates: This field displays the number of microplates required and depends on the plate format
(Section 8.5) and number of Wells.
• Min. Well Volume: This field displays the minimum required well or tube volume. Min. Well Volume
must be less than or equal to the ml/well or ml/tube volume selected in the "Plate Options" panel
(see Section 8.5) or a "Well overflow!" warning will appear. If a "Well overflow!" warning is displayed,
either use a microplate with a larger well volume or reduce the Multiple cuts/object value in the
“Cut Options panel” (see Section 8.4).