Bio-Rad EXQuest Spot Cutter - Manual (Page 42)

5.3.2 Identifying Re-cuts from a Gel Image
1. When the cut run is finished, return to the "Manual Excision Tool" screen and click Confirm Cuts to
acquire a confirmation image.
2. Closely examine the gel image for spots that were not picked up. Use the zoom and transform
functions on the toolbar to get a better view of the gel.
3. When a missed spot is located, click in the "Manual Excision Tool" window and then right click on
the missed cuts cut circle to the recut it.
4. Switch back to the Confirm Cuts image and repeat steps 2 and 3 until all missed spots are selected
to be re-cut.
5. Click Begin/Resume Cuts to perform the re-cuts.
MANUAL EXCISION TOOL
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