Bio-Rad EXQuest Spot Cutter - Manual (Page 31)

d. Use the Raise/Lower slider to adjust the tip height. Typically, the gel tip should be placed about
0 to 1 mm into the well and the Membrane cutting tip 1–2 mm into the well. Note that the
membrane cutting tip must be in contact with water in the wells to release membrane plugs. To
ensure that the cutting tip touches water when releasing the membrane plugs, fill the microplate
with the amount of water that will be used during the cut run and then calibrate the microplate
tip height so it just touches the water.
e. Press Next to save the calibration.
f. To calibrate another plate format, click Yes and then Next (see Figure 3-12). To continue to the
Wash Station Position Calibration step, click No and then Next. Press Cancel to quit the
calibration. The calibration completed in all previous steps will be saved.
Figure 3-12. Plate position calibration: calibrate another microplate format.
11. Wash Station Position Calibration. The wash station position calibration step is used to center the
cutting tip over the wash ports.
a. Use the Left/Right and Front/Back slider to center the cutting tip over the wash port. The gel tip
is centered over the left wash port (see Figure 3-13) and the membrane tip is centered over the
microtube in the left membrane tip wash port. Click on the slider bar to move the tip in small
increments or click and drag the slider to make larger adjustments. When the tip is centered,
check Lower head to adjust eject height to lower the cutting tip into the well. Use the sliders to
center the tip into the well.
EXQUEST CALIBRATION WIZARD
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