Bio-Rad Experion Protein Analysis Kits - Manual (Page 14)

3.1 Set Up the Electrophoresis Station
1. If needed, perform a deep cleaning of the electrodes (see Appendix B for instructions).
2. Power on the computer and then power on the Experion electrophoresis station by pushing the
green button in the center of the front panel. The steady green LED above the button indicates that
the unit is on.
3. Launch Experion software. If the instrument and computer are communicating properly:
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A green dot and the last 4 digits of the instrument serial number appear in the lower right corner
of the software screen
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The electrophoresis station icon appears in the upper left corner
When there is no connection, these indicators are absent and a grayed-out instrument icon appears
in the upper left corner of the software screen.
3.2 Equilibrate the Kit Reagents
1. Set a heating block or water bath to 95–100°C. You will use this heating block to denature the
samples and Pro260 ladder later in the protocol.
2. Equilibrate the following kit reagents to room temperature for ~15–20 min:
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Pro260 stain (blue cap)
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Pro260 sample buffer (yellow cap)
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Pro260 ladder (red cap)
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2 tubes Pro260 gel (green cap)
3. Vortex the contents of each tube and briefly centrifuge the solutions to the bottoms of the tubes.
Make sure the Pro260 stain solution (blue cap) is thawed before proceeding.
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If the gel-stain solution (GS) and filtered gel (G) were prepared previously, equilibrate them to room
temperature. Use the GS and G within 1 month of preparation. After 1 month, refilter them before
use. Keep both the G and GS at room temperature and covered until ready for use.
3.3 Filter the Gel and Prepare the Gel-Stain Solution
1. Prepare the GS by adding 20 µl Pro260 stain (blue cap) to a tube of Pro260 gel (green cap, 520 µl).
Vortex the GS for 10 sec at the highest setting and then spin it down briefly in a microcentrifuge.
2. Transfer the GS to a spin filter, and transfer the contents of the other Pro260 gel (green cap) into
another spin filter. Label and date the tubes.
3. Centrifuge both spin filters for 5 min at 10,000 × g. Inspect the tubes to ensure all of the gel passed
through the filters, and then discard the filters. Cover the GS with foil.
Experion Automated Electrophoresis System