1 product description – Bio-Rad Experion Protein Analysis Kits User Manual

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Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com

1.1 Product Description

The Experion Pro260 analysis kit is used for protein analysis with the Experion automated
electrophoresis system (Figure 1.1). The Experion system employs LabChip microfluidic technology
to automate protein and nucleic acid electrophoresis by integrating separation, detection, and data
analysis within a single platform. The Experion automated electrophoresis system uses smaller sample
and reagent quantities than standard analysis methods, and it accomplishes analysis in a single
30-minute, automated step.

The Experion Pro260 analysis kit supplies the microfluidic chips, reagents, and instructions required to
separate and analyze 10–260 kD proteins under denaturing conditions. The sensitivity of the Experion
Pro260 analysis kit is comparable to (though sometimes more sensitive than) colloidal Coomassie Blue
staining of SDS-PAGE gels. Each Experion Pro260 chip can analyze up to 10 samples.

For details about how the Experion Pro260 kit analyzes proteins, refer to Appendix A in this manual.

Fig . 1 .1 . The Experion system . The system includes the following components: 1) automated electrophoresis
station, 2) priming station, 3) vortex station used for nucleic acid analysis only, 4) system operation and data analysis
tools (software), and 5) analysis kits, which include the (a) chips and (b) reagents for protein (Pro260 kit), standard-
sensitivity RNA (StdSens kit), high-sensitivity RNA (HighSens kit), and DNA (DNA 1K and 12K kits) analyses.

Register your Experion system in order to ensure you receive important updates on software,
tech notes, and manuals. Upon installation, a dialog will provide registration instructions.

Experion Automated Electrophoresis System

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