Bio-Rad Experion Protein Analysis Kits User Manual
Page 61
Table C.9. Detergents and reductants with severe effects on Experion Pro260 separation or analysis.
Detergent/Reductant
Concentration
Effect(s)
ASB-14
2%
Enlarged system peak shifts to right; 10 kD peak is missing
n-Dodecyl-
b-D-maltoside
1%
Enlarged system peak shifts to right; 10 kD peak is missing
NP40
2%
Enlarged system peak shifts to right; 10 kD peak is missing
SB3-10
2%
Enlarged system peak shifts to right; 10 kD sizing affected
Triton X-100
1%
Enlarged system peak shifts to right; 10 kD peak is missing
Tween-20
1%
Enlarged system peak shifts to right; 10 kD peak is missing
Tween-80
1%
Enlarged system peak shifts to right; 10 kD peak is missing
Zwittergent 3-14
1%
Enlarged system peak shifts to right; 10 kD peak is missing
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Experion Pro260 Analysis Kit