Bio-Rad Experion Protein Analysis Kits - Manual (Page 58)

Appendix C: Chemical Compatibility
For best results, the starting protein sample (undiluted) should contain components at concentrations
less than or equal to the values indicated in the tables below. If concentrations are above these values,
dilute the samples accordingly.
Buffers
Table C.1. Buffers with no observable effect on Experion ™ Pro260 separation or analysis at or below the
concentrations listed.
Buffer Concentration
B-PER (Pierce) 0.5x
BugBuster (Novagen) 1x: BugBuster and benzonase nuclease
CPEB (ReadyPrep ™ protein extraction 0.5x
kit, cytoplasmic/nuclear)
GST binding/wash buffer (Novagen)
0.5x
His elution buffer (Pierce) 0.5x
His wash buffer 1 (Pierce) 0.5x
His wash buffer 2 (Pierce) 0.5x
IEF cathode buffer 1.5x: 30 mM Lysine, 30 mM arginine
IMAC elution buffer 1x: 50 mM KH
2
PO
4
, 150 mM NaCl, 250 mM imidazole, pH 8.0
IMAC lysis buffer 1x: 25 mM KH
2
PO
4
, 150 mM NaCl, pH 8.0
IMAC wash buffer 1 1x: 100 mM KH
2
PO
4
, 600 mM KCl, 10 mM imidazole, pH 8.0
IMAC wash buffer 2 1x: 100 mM KH
2
PO
4
, 600 mM KCl, 20 mM imidazole, pH 8.0
LB medium 0.5x: 1% Tryptone, 0.5% yeast extract, 1% NaCl
M1 (ReadyPrep protein extraction kit, 0.5x
membrane I)
M2 (ReadyPrep protein extraction kit, 0.5x
membrane I)
M-PER
0.5x
PBS 1.5x: 15 mM Sodium phosphate, 225 mM NaCl, pH 7.5
PBS-Tween 1x: 10 mM Sodium phosphate, 150 mM NaCl, 0.05% Tween, pH 7.2
S1 (ReadyPrep protein extraction kit, 0.5x
signal)
Tricine sample buffer
0.5x: 100 mM Tris-HCl, pH 6.8, 1% SDS, 20% glycerol, 0.02% Coomassie Brilliant Blue
Tris-borate-EDTA (TBE) 1.5x: 133 mM Tris, 133 mM boric acid, 3 mM EDTA, pH 8.3
Tris/CAPS 1.5x: 90 mM Tris, 60 mM CAPS, 0.15% SDS pH 9.6
Tris/glycine 1.5x: 37.5 mM Tris, 288 mM glycine, pH 8.3
Tris/glycine/SDS 1.5x: 37.5 mM Tris, 288 mM glycine, 0.15% SDS, pH 8.3
Tris/Tricine/SDS 1.5x: 150 mM Tris, 150 mM Tricine, 0.15% SDS, pH 8.3
Urea/phosphate/NaCl buffer 0.5x: 4 M Urea, 25 mM sodium phosphate, 150 mM NaCl, pH 8
Criterion ™ XT MOPS buffer 1.5x
Criterion XT sample buffer 1.5x
Criterion XT Tricine buffer 1.5x
Zymogram sample buffer 0.5x: 31.3 mM Tris-HCl, pH 6.8, 2% SDS, 12.5% glycerol, 0.005% bromophenol blue
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Experion Automated Electrophoresis System