Bio-Rad Experion Protein Analysis Kits - Manual (Page 60)

Table C.5. Chemicals with mild effects on Experion Pro260 separation or analysis.
Chemical Concentration Effect(s)
Acetone 15% Quantitation affected
Bio-Lyte ® 3/10 0.75% Quantitation affected
Guanidine HCl 25 mM Enlarged system peak shifts to right; 10 kD sizing affected
KCl 1.5 M Quantitation affected
MES 50 mM, pH 6.0 Quantitation affected
MOPS 100 mM, pH 7.2 Quantitation affected
PEG 0.50% Quantitation affected
Propylene glycol 50% Enlarged system peak shifts to right; 10 kD sizing affected
Sucrose 200 mM Quantitation affected
Table C.6. Chemicals with severe effects on Experion Pro260 separation or analysis.
Chemical Concentration Effect(s)
Ammonium sulfate 1.5 M Protein precipitation
Guanidine isothiocyanate 1 M Protein precipitation; no markers appear
Guanidine HCl 1 M Protein precipitation; no markers appear
HCl 150 mM Protein precipitation; markers missing
Pluronic F-68 0.1% Protein smears
Detergents and Reductants
Table C.7. Detergents and reductants with no observable effects on Experion Pro260 separation or analysis.
Detergent/Reductant Concentration
ASB-14 0.10%
CHAPS 0.50%
CHAPSO 0.10%
Deoxycholate 1.0%
Octyl-thioglucopyranoside 0.50%
Tributylphosphine (TBP) 7.5 mM
Triton X-100 0.40%
Tween-20 0.50%
Table C.8. Detergents and reductants with mild effects on Experion Pro260 separation or analysis.
Detergent/Reductant Concentration Effect(s)
ASB-14 0.5% Enlarged system peak shifts to right; 10 kD sizing affected
CHAPS 1% Enlarged system peak shifts to right; 10 kD sizing affected
CHAPSO 0.2% Enlarged system peak shifts to right; 10 kD sizing affected
Deoxycholate 2% Enlarged system peak shifts to right; 10 kD sizing affected
LDS 3% Enlarged system peak shifts to right; 10 kD sizing affected
NP40 0.5% Enlarged system peak shifts to right; 10 kD sizing affected
SB3-10 0.1% Enlarged system peak shifts to right; 10 kD sizing and quantitation
affected
SDS 1% Quantitation affected
Tween-20 0.75% Enlarged system peak shifts to right; 10 kD sizing affected
Zwittergent 3-14 0.10% Enlarged system peak shifts to right; 10 kD sizing affected
β-Mercaptoethanol 1.5 M Quantitation affected
DTT 150 mM Quantitation affected
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Experion Automated Electrophoresis System