Bio-Rad Experion Protein Analysis Kits - Manual (Page 44)

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Fig . 6 .4 . Manual integration mode indicates the beginning and end of peak baselines
with blue dots .
6.9 Manual Peak Integration
This function enables definition or refinement of peak areas for the ladder and all sample lanes.
1. Select a sample and click or select Analysis > Manual Integration to start manual integration.
2. Adjust the baseline by clicking and dragging the dots at the ends of the peaks (Figure 6.4).
If a peak in the electropherogram is not detected by the software, right-click on that peak and select
Add Peak. Zoom in on the peak for a better view of the baseline.
3. Save any changes by selecting File > Save Run As (use a new file name), or revert to the original
baseline and integration by clicking .
Manual integration applies to individual sample wells. Moving back to automated integration loses
changes made during manual integration unless those changes are saved.
Experion Automated Electrophoresis System