Bio-Rad Experion Protein Analysis Kits - Manual (Page 63)

Normalization
Alignment of protein peak migration times across
different sample wells of the same chip; the lower
and upper markers are used by Experion software
for this purpose
Overlay
Function of Experion software that allows different
electropherograms to be superimposed for easier
comparison
Peak
Signal in an electropherogram generated
and identified by the peak-find settings to be
significantly different from the baseline
Priming
Filling the microchannels of the microfluidic chip
with gel-stain solution in preparation for analysis
Priming station
Instrument used to prepare chips for sample
analysis; applies pressure to the priming well
of the microfluidic chip and fills the network of
microchannels with the gel-stain solution
Pro260 ladder
Modified version of the Precision Plus Protein
™
standards that has been optimized for automated
electrophoresis on the Experion system; contains
9 purified recombinant proteins of 10–260 kD
Project folder
User-defined folder containing multiple run files
comprising a particular project
Project tree
Hierarchical tree of all saved projects and the data
files they contain
Quantitation
Determination of the concentration of a protein in
a sample
Reducing
Sample condition that includes reducing agents
for the reduction of disulfide bonds
Relative quantitation
Method of quantitation in which concentrations
are determined against a single-point internal
standard
Results table
Tab that displays analysis results, settings, and
parameters
Results and settings
Portion of the Experion software user interface
dedicated to display of tabulated analysis results
and analysis settings and parameters
Run file
File containing data for a single run
Single-well view
Function of Experion software that allows an
electropherogram from a single well to be viewed
Sizing
Determination of the molecular mass of a protein
Spin filter
Polypropylene spin tubes with filter membranes
used to remove particulate matter from the
gel-stain (GS) and gel (G) by centrifugation
System peaks
Cluster of signals generated by small molecules
that interact with fluorescent detergent micelles;
not considered in the concentration estimation
Tree view
Portion of the Experion software user interface
dedicated to display of the project tree; used to
locate and manipulate data files and organize runs
into projects
Upper marker
260 kD protein included in the Experion Pro260
sample buffer and used to normalize the
separation of proteins across all wells in the chips;
also used as an internal standard for relative
quantitation by default
User-defined standard
Protein (10–260 kD) added to each sample at a
known concentration and used for quantitation
Virtual gel
Densitometric, gel-like image generated from
electropherogram data and displaying peaks as
bands; displayed in the gel view
Vortex station
Instrument used to mix RNA or DNA samples and
loading buffers (supplied in the kits) in the chip
wells; used for nucleic acid applications only
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Experion Pro260 Analysis Kit