Bio-Rad Experion Protein Analysis Kits - Manual (Page 59)

Table C.2. Buffers with mild effects on Experion Pro260 separation or analysis.
Buffer Concentration Effect(s)
IEF anode buffer 1.5x: 10.5 mM phosphoric acid Quantitation
Laemmli buffer 0.5x: 31.3 mM Tris-HCl, pH 6.8, 1% SDS, Quantitation
12.5% glycerol, 0.005% bromophenol blue,
2.5% b-mercapotethanol
PSB (ReadyPrep protein extraction kit, 0.5x Quantitation
membrane I)
RIPA buffer
1x: 150 mM NaCl, 1.0% NP40, 0.5%
sodium deoxycholate, 0.1% SDS, 50 mM Tris Enlarged system peak shifts to
right; 10 kD sizing affected
TBS 1.5x: 30 mM Tris, 750 mM NaCl pH 7.4 Quantitation
Table C.3. Buffers with severe effects on Experion Pro260 separation or analysis.
Buffer Effect(s)
Rehydration buffer (ReadyPrep 2-D starter kit) Enlarged system peak shifts to right; 10 kD peak is missing, sizing affected
S2 (ReadyPrep protein extraction kit, signal) Enlarged system peak shifts to right; 10 kD peak is missing, sizing affected
Reagent 2 (ReadyPrep sequential extraction kit) Enlarged system peak shifts to right; 10 kD peak is missing, sizing affected
Reagent 3 (ReadyPrep sequential extraction kit) Enlarged system peak shifts to right; 10 kD peak is missing, sizing affected
Total protein extraction buffer
Enlarged system peak shifts to right; 10 kD peak is missing, sizing affected
Criterion XT MES buffer (1.5x) 10 kD peak is missing
Chemicals
Table C.4. Chemicals with no observable effects on Experion Pro260 separation or analysis at or below the
concentrations listed.
Chemical Concentration Chemical Concentration
Acetonitrile 30% MeOH 15%
Ammonium sulfate 250 mM Na
3
VO
4
5 mM
Arginine 500 mM NaCl 250 mM
DMSO 15% NaF 50 mM
EDTA 10 mM NaHCO
3
50 mM, pH 8
EGTA 150 mM NaN
3
0.75%
Ethanol 30% NaOAc 50 mM, pH 4
Glucose 0.5 M NaOH 150 mM
Glycerol 35% NDSB201 1%
Glycine 1 M Sodium carbonate 150 mM, pH 11.5
Guanidine isothiocyanate 25 mM Succinic acid 100 mM
HEPES 50 mM, pH 7 Tetramethylammonium chloride 1 M
Imidazole 0.5 M, pH 7 Trehalose 5%
KCl 250 mM Tris base 1.5 M
MgCl
2
150 mM Urea 4.5 M
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Experion Pro260 Analysis Kit