Bio-Rad Experion Protein Analysis Kits - Manual (Page 29)

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4.3 Using Results and Settings to View and Annotate Data
The following options appear under the results and settings tabs:
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Results — summarizes analysis data for all detected peaks. The data in this table can be
exported (see Section 4.5.2, Exporting Data Files to Other Applications). To view the results
of a particular sample, click the electropherogram in the all-wells view or lane in the gel
view, or select that sample from the project tree. Alternatively, use the arrows in the main
screen toolbar to navigate through the samples. See below for instructions on how to
customize the types of data shown under this tab
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Run Summary — displays a run summary for all wells; right-click to export the data
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Protein Search Result — summarizes user-defined known proteins: lists the samples
that contain a peak that fits with the search of size, or molecular mass as defined
under the Protein Search Criteria tab and performs statistical calculations for the size,
concentration, and percentage of total protein for each peak identified
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Protein Search Criteria — used to compare statistics for specific proteins in the samples
(see Section 6.1, Designating and Searching for Specific Proteins for information on how to
designate and then search for known proteins)
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Run Info — used to enter sample names and comments after a run is finished (see below
for instructions)
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Settings — used mostly for troubleshooting, but can be used to edit the parameters used
to identify peaks (see Section 6.5, Changing Peak Finding Parameters, or the Experion
software Help file for more details about these settings)
To customize the types of data shown under the Results tab:
1. Right-click on any of the column headers in the Results tab (or click the arrow to the left of the
column header). The Column Selector dialog opens.
2. Use the arrow keys to show, hide, or change the order of display of any of the available headers.
For detailed explanations of each setting, refer to the software Help file.
3. Click OK to save the changes, or click Cancel to close the dialog without saving the changes.
Under the Results tab, total concentration data appear at the bottom of the table. This calculation
represents the total protein concentration of the original sample. See Bio-Rad bulletins 5299, 5784,
and 5423 to learn more about comparing these calculations to data gathered by other methods.
To enter sample names and descriptions:
1. Click the Run Info tab and then click on the plus sign (+) next to Sample Information.
2. Click on any active cell, enter the sample names and descriptions, and click Apply. Click
File/Save to save the changes. The sample name appears above its electropherogram.
To enter other information about the experiment:
1. In the Run Info tab, click the plus sign (+) next to the Study Information and/or System
Information area(s).
2. Double-click in any blank field, enter the information, and click Apply. Click File/Save.
Experion Pro260 Analysis Kit