Bio-Rad Experion Protein Analysis Kits - Manual (Page 7)

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1.2 Kit Components
Table 1.1. Components of the Experion Pro260 analysis kit.
Item Description Volume (per Vial) 10-Chip Kit
Pro260 chip Microfluidic chips used for protein separation 10 chips
Cleaning chip Chip used for cleaning electrodes 1 chip
Pro260 gel Proprietary polymeric sieving matrix 520 µl 3 vials
Pro260 stain Proprietary fluorescent dye 45 µl 1 vial
Sample buffer Buffer for protein sample preparation; contains a 1.2 kD 400 µl 1 vial
lower marker and 260 kD upper marker for alignment of
samples to the Pro260 ladder
Pro260 ladder Protein standard containing 9 purified recombinant proteins 60 µl 1 vial
of 10–260 kD and optimized for automated electrophoresis
on the Experion system
Spin filters Used for filtering reagents during sample preparation 3 filters
1.3 Storage Conditions
Table 1.2. Storage conditions.
Item Storage Shelf Life
Experion Pro260 reagents 4ºC See expiration date on packaging
Experion Pro260 chips Ambient See expiration date on packaging
Gel-stain solution (GS, prepared) 4ºC 1 month from filtration (can be refiltered once)
Gel (G, prepared) 4ºC 1 month from filtration (can be refiltered once)
1.4 Specifications
Number of sample wells per chip 10
Sample volume required 4 µl
Total run time ~30 min per chip
Protein sizing range 10–260 kD
Limit of detection 2.5 ng/µl carbonic anhydrase (10 ng total) in 1x PBS; similar to
colloidal Coomassie Blue G-250 stain
Linear dynamic range 5–2,000 ng/µl (bovine serum albumin in 1x PBS)
Experion Pro260 Analysis Kit