Bio-Rad Experion Protein Analysis Kits - Manual (Page 50)

Error Probable Cause Recommended Action
Baseline is fluctuating (for
example into very negative
and positive values) Air bubbles are interfering with electrical
contact in one or more of the wells Stop the run, remove the chip, and remove and replace
solution in affected wells
Chip is not completely primed Prime and load a new chip
Samples are too concentrated; protein levels
are out of the range of the assay Dilute the samples and repeat the analysis
7.3 Contacting Technical Support
If you are having problems with a run and would like the Bio-Rad Technical Support team to review your
data, please submit the following files to LSG_TechServ_US@bio-rad .com for analysis:
n
Run file (C:\Program Files\Bio-Rad Laboratories\Experion Software\Data\<daily folder
name>\<run name>.bdf)
n
Run log (C:\Program Files\Bio-Rad Laboratories\Experion Software\Data\<daily folder
name>\<run name>.log)
n
Packet file (C:\Program Files\Bio-Rad Laboratories\Experion Software\Data\<daily folder
name>\Packet\<run name>.txt)
n
System log (C:\Program Files\Bio-Rad Laboratories\Experion Software\Backup\Version X\
Experion_System.log or .txt)
n
Lot numbers of the Pro260 chips and the Experion Pro260 analysis kit
To export the run file, log file, and packet file, select the run(s) in the tree view and click Export Selected
Runs. The software exports the files into folders with the corresponding project name(s).
46 Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
Experion Automated Electrophoresis System