Bio-Rad Econo Gradient Pump Kits User Manual

Page 26

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Each field may be selected with the corresponding soft key. The
arrow keys are then used to increment or decrement the value.
Pressing the Step key or the Next key will accept the displayed
values and advance to the next step. The program end occurs when
either 6 steps have been entered, or two identical steps (pressing
Step twice) are entered sequentially.

If an auxiliary valve is present, a screen will appear after
programming is completed.

If an SV-3 diverter valve is connected, then a fraction collection
window will appear. The units will be either in minutes (m) or
volume (ml.)

from COLLECT to

2m 10m

In this example, the valve will be in the DIVERT position from t =
2min to t = 10min, diverting the eluant stream to the fraction
collector. All other times, it will be in the WASTE position, diverting
the eluant stream to waste. Fraction collection size is programmed
directly at the fraction collector faceplate.

If a splitter valve is connected, then a split per cent window appears

splitter

25%

In this example, 25% of the eluant stream is split into another
receptacle such as a microplate or secondary fraction collector.

Pressing the Step key scrolls through each step, illuminating the LED
for that step and allowing the user to observe and edit the program.
At the AUX Valve screen, LEDs for all the programmed steps are
illuminated. The program may be deleted using the ERASE function
in Setup mode.

System Operation

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