Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 11)

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Figure 1A depicts six time points. The adsorbed
concentration and the liquid phase concentration at
equilibrium are represented by q
6
and c
6
, respectively.
The time to reach equilibrium is represented as t
6
.
Figure 1B shows three operating lines with distinct
slopes. The slopes represent different values of β
(phase ratio). Line I has the highest phase ratio,
followed by line II and line III.
To work at the saturation capacity (q
max
) use a small
volume of media and a relatively high sample volume,
which leads to a high phase ratio (Figure 1B, β
I
).
Working at the saturation capacity may be of interest
when performing capacity (capture) studies.
Conversely, to operate under the saturation capacity
a larger volume of media should be used, which
results in a lower phase ratio. This is recommended
when performing wash and elution studies
(Figure 1B, β
I
, β
II
). This reasoning assumes that
isotherm data for a given system are available.