Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 9)

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To use Eq. 3a, only the flowthrough concentration
must be measured. Conversely, using Eq. 3c
requires the solute concentration in the elution and
regeneration steps be input. Thus, in order to use
Eq. 3c, the experimental conditions should first be
optimized to guarantee the total recovery of the
solute.
Note: Calculating the amount of bound solute using
flowthrough data (C
FT
) and Eq. 3a is a faster and
relatively simple approach.
Two process performance indicators can be
calculated using the amount of bound solute (m
bound
).
These indicators are recovery, Eq. 4a, and % yield
Eq. 4b:
bound unbound
loaded
Recovery 100%
m m
m
+
=
eluted
bound
Yield 100%
m
m
=
2.3 Phase Ratio ( β)
The phase ratio is defined as the ratio of the liquid to
solid phase in a given well, represented as follows:
0
ads
V
V
β =
Where β represents the phase ratio. The phase ratio
is an important variable because: (1) it determines the
slope of the operational line in a given isotherm; (2) it
determines the level of depletion of the solute liquid
phase concentration; and (3) it directly affects the
magnitude of the experimental error incurred during
HT chromatographic experiments.
Eq. 4a
Eq. 4b
Eq. 5