Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 6)

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Section 1
Introduction
The advent of high-throughput (HT) experimentation has
revolutionized the conventional chromatographic process
development paradigm. Traditionally, chromatographic
process development data are obtained using column
experiments alone.
These types of experiments usually consume significant
amounts of sample, limiting the number of experiments
that can be performed to define a given process design
space.
Consequently, this design space is considered a
nonoptimal space. HT experimentation substantially
overcomes these limitations by allowing the execution
of experiments in a miniaturized parallel format, yielding
significant savings in time and material costs. 1-2
To achieve this, HT experimentation utilizes microtiter
plates and multichannel liquid dispensing tools (such as
multichannel pipets and pipetting robotic platforms).
Two types of microtiter plates are commonly required,
a filter plate containing the chromatographic media
and a collection plate used to collect the sample. HT
experimentation chromatographic experiments have been
successfully used for isotherm determination, 3 method
development and optimization, 4-5 and resin screening. 6 HT
experimentation can be used with design of experiments
(DoE), thereby becoming an essential tool to be employed
in quality by design (QbD) and process analytical
technology (PAT) approaches. 7-8
This instruction manual describes how to use Foresight™
prepacked filter plates chromatography media.