Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 3)

Contents
Section 1 Introduction 1
Section 2 Theoretical Background 2
2.1 Batch Adsorption 2
2.2 Mass Balance 2
2.3 Phase Ratio (
β) 4
2.4 Adsorption Isotherm 5
2.5 Uptake Curve 8
2.6 Contact Time 9
2.7 Design of Experiments 10
2.8 Scaling up from Plates to Columns 10
Section 3 High-Throughput Experiments 11
3.1 Manual Workflow 11
3.2 Types of Studies 12
3.2.1 Screening 12
3.2.2 Adsorption Isotherm 13
3.2.3 Binding Kinetics 15
3.3 General Considerations 17
3.3.1 Sample 17
3.3.2 Media Plate Handling 17
3.3.3 Liquid transfer and Buffer
Preparation 17
3.3.4 Liquid Removal 17
3.3.5 Mixing 18
3.4 Reliability of the HT experimentation
Data: Error Analysis and
Error Propagation 19
3.5 Equipment and Materials Required for
HT Experiments 22
3.6 Detailed Experimental Protocol 22
3.6.1 Media Plate Preparation 22
3.6.2 Plate Equilibration 23
3.6.3 Sample Loading 24
3.6.4 Washing Weakly Bound Material 25
3.6.5 Elution 25