Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 27)

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3.5 Equipment and Materials Required for HT
Experiments
In addition to the Foresight chromatography media
plates the following equipment is required:
(a) 96-well collection plates, (b) 8- or 12-channel
pipets, (c) microplate shaker that can mix at
1100 rpm, (d) bucket centrifuge equipped with a
rotor for microtiter plates, and (e) microtiter plate
spectrophotometer.
HT experimentation analytics must accommodate
and be optimized for a large number of samples of
relatively small volume. Accessibility to an HPLC
system equipped with a microtiter plate autosampler
will increase the analytical throughput.
3.6 Detailed Experimental Protocol
3.6.1 Media Plate Preparation
a) Resuspend the medium to guarantee that
media adhered to the plate’s top seal returns
to the well. Either one of the following two
methods may be followed to ensure proper
resuspension of the media:
• Gently tap filter plate on benchtop 20
times; or
• Centrifiguge plate at 100 x g for 60–120
seconds
b) Remove the media plate’s bottom seal and
place the media plate on top of its
corresponding 96-well collection plate. Next,
peel off the top seal while holding the media
plate firmly.
c) Because the liquid storage solution does
not contain valuable solutes, remove the
solution by either vacuum filtration or
centrifugation.