Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 16)

Technical Support: 1-800-424-6723 • www.bio-rad.com 11
Section 3
High-Throughput Experiments
3.1 Manual Workflow
A manual workflow for HT experimentation starts
with plate equilibration, followed by sample loading,
incubation under mixing, and collection of unbound
solute. These stages are common for uptake
and isotherm experiments. For wash and elution
experiments, the media plate (containing bound
solute) is placed on top of a new collection plate.
After the media plate is washed, the collected wash
is analyzed. Next, the media plate is placed on top
of a new collection plate for elution. The number of
collection plates will be determined by the number of
elution steps desired. Figure 2 shows the commonly
encountered stages in a HT experimentation manual
workflow. The schematic starts with sample loading,
as it is assumed that the media plate has been
previously equilibrated.
Fig. 2. Schematic representation of a HT
experimentation manual workflow.