Od c m, Od m m, C v cv qv – Bio-Rad Foresight™ Chromatography Columns, Prepacked User Manual
Page 25: Vq c c v
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Corr
P
OD
C
m
=
Corr
2
2
Corr
2
1
P
C
OD
m
OD
m
m
σ
σ
σ
=
+ −
Where C
p
and σ
p
represent the calculated protein
concentration and its corresponding uncertainty,
respectively. m represents the slope of the calibration
line (protein concentration vs. absorbance) and σ
m
its
corresponding uncertainty.
Load L
L
Media
C V
CV
qV
=
+
(
)
L
Load
Media
V
q
C
C
V
=
−
Load
2
2
L
L
Media
Media
q
C
C
V
V
V
V
σ
σ
σ
=
+ −
Where C
Load
represents the protein load
concentration, C the unbound protein concentration,
and q the bound protein concentration. V
L
and
V
Media
represent the volumes of liquid and media,
respectively. All
σ represent the uncertainty in each
quantity.
In sum, using error analysis, the uncertainties of
the measurable quantities are used to estimate
the uncertainty of the calculated quantity. These
expressions can be used, for example, to assess the
experimental error of HT experimentation isotherm
data in both coordinates, as shown in Figure 5.
Eq. 10a
Eq. 10b
Eq. 11a
Eq. 11b
Eq. 11c