Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 21)

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Loading Order first last
time (min) t
1
t
2
t
3
… t
10
t
11
t
12
C
0
(mg/mL) 1 2 3 … 10 11 12
A
B
C
D
E
F
G Δt = t
final
-t
inital
/(N
t
-1)
(B) H N
t
: Number of time points
C
1
C
2
Fig. 4. HT uptake experiments. A, loading sequence
of the plate. The color gradients indicate the level of
saturation or depletion of the liquid and solid phases.
The blue and green represent the liquid and media,
respectively. In this example only three data points
are shown, t
1
is the longest incubation time and t
3
the
shortest; B, example of plate configuration for HT uptake
experiments as a function of the initial solute concentration
(C
0
).
B