Time points along the operating line; c, Initial concentration; q, Solid phase concentration at equilibrium; c – Bio-Rad Foresight™ Chromatography Columns, Prepacked User Manual

Page 12: Liquid phase concentration in equilibrium with q

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Fig. 1. Schematic representation of an adsorption
isotherm. A, operating line describing the trajectory
followed during the adsorption process, from the initial
state to the equilibrium state. Dashed line: operating
line; t

1…6

: time points along the operating line; C

0

: Initial

concentration; q

1

: solid phase concentration at equilibrium;

c

1

: liquid phase concentration in equilibrium with q

1

;

q

max

: maximum binding capacity; B, operating lines with

distinct slopes. Their different slopes are the result of using
different phase ratios during the adsorption step.

β

I

>

β

II

>

β

III

.

A

B

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