Time points along the operating line; c, Initial concentration; q, Solid phase concentration at equilibrium; c – Bio-Rad Foresight™ Chromatography Columns, Prepacked User Manual
Page 12: Liquid phase concentration in equilibrium with q
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Fig. 1. Schematic representation of an adsorption
isotherm. A, operating line describing the trajectory
followed during the adsorption process, from the initial
state to the equilibrium state. Dashed line: operating
line; t
1…6
: time points along the operating line; C
0
: Initial
concentration; q
1
: solid phase concentration at equilibrium;
c
1
: liquid phase concentration in equilibrium with q
1
;
q
max
: maximum binding capacity; B, operating lines with
distinct slopes. Their different slopes are the result of using
different phase ratios during the adsorption step.
β
I
>
β
II
>
β
III
.
A
B
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