YSI 600LS - User Manual - Manual (Page 54)

Sondes Section 2
YSI Incorporated Environmental Monitoring Systems Operations Manual 2-48
RHODAMINE WT 2-POINT
Select Rhodamine from the Calibrate Menu and then 2-2-Point.
NOTE: One standard must be 0 ug/L in rhodamine WT, and this standard must be calibrated first.
To begin the calibration, place the correct amount (see Tables 1-8) of 0 standard (clear deionized or
distilled water) into the calibration cup provided with your sonde and immerse the sonde in the water.
Input the value 0 ug/L at the prompt, and press Enter. The screen will display real-time readings that will
allow you to determine when the readings have stabilized. Activate the wiper 1-2 times by pressing 3-
Clean Optics as shown on the screen, to remove any bubbles. After stabilization is complete, press Enter
to “confirm” the first calibration and then, as instructed, press Enter to continue.
Dry the sonde carefully and then place the sonde in the second rhodamine WT standard (100 ug/L is
recommended) using the same container as for the 0 ug/L standard. Input the correct rhodamine WT
concentration in ug/L, press Enter, and view the stabilization of the values on the screen in real-time. As
above, activate the wiper with the “3” key or manually rotate the sonde to remove bubbles. After the
readings have stabilized, press Enter to confirm the calibration and then press Enter to return to the
Calibrate menu.
Thoroughly rinse and dry the calibration cups for future use.