Luminex IS Version 2.3 - Manual (Page 52)

Luminex IS Software Manual for Version 2.3
x
MAP Technology
42 PN 89-00002-00-110 Rev. B
Set Luminex XYP
Instrument Heater
Temperature
Refer to your assay kit instructions to see if the assay needs to be
analyzed at a particular temperature. If the instructions indicate that
the Luminex XYP instrument heater is needed, set the heater to the
specified heat setting. The user definable heater range is 35°C to
60°C. Use the heater only with the heater block in place.
Luminex recommends using a Costar® Thermowell® thin-wall
polycarbonate 96-well plate (nonskirted), model P over the heater
block sent with the Luminex System. Do not use standard 96-well
microtiter plates if you are using the heater block.
Any temperature that you set remains in effect until you set another
temperature or turn off the Luminex XYP instrument plate heater or
exit the software.
The system displays the target temperature in the box below the Turn
ON button. Before the heater block temperature reaches the new
temperature setting, the XYP Heater Temperature thermometer is
red. Upon reaching the target temperature, the thermometer turns
green. See page 9 for more information about the system monitor.
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To set the Luminex XYP instrument heater temperature:
1. Click Eject/Retract to eject the plate holder.
2. Set the Luminex XYP heater block on the plate holder.
3. Click Eject/Retract to retract the plate holder.
4. In the Temperature and Gauges area of the Run Batch tab,
click Turn ON. The light on the button turns green and the
thermometer fluid turns red as it raises to reach the target
temperature.
5. Use the up and down arrows beneath the target temperature box
to set the temperature you want the Luminex XYP instrument
heater block to maintain. The user definable heater range is 35°C
to 60°C.
6. Wait for the heater to reach your selected temperature and
stabilize before processing samples. The thermometer turns
green when the temperature is stabilized.
Calibration Procedures
Calibrator xMAP microspheres are used to normalize the settings for
the reporter channel, both classification channels, and the doublet
Warning: The heater plate of the Luminex XYP instrument is hot
when in use and may cause burns. Do not touch the heater plate.