Multichannel Systems HiClamp Manual - Manual (Page 19)

HiClamp
13
For washing the cell between two measuring cycles there is a washing station placed
between the well plates.
The well plate on the right hand side is called “Feeder Plate” and contains the oocytes to be
tested. The well plate on the left hand side is called “Compound Plate” and contains the solutions
of interest. Each compound well should be filled with 230 μl. Additionally a micro stirrer is placed
inside which is moved by magnetic forces.
Immersion of the oocyte in the compound liquid is not sufficient to ensure a quick access of the
drug to the cell membrane. Passive diffusion of the molecule of interest alone to the membrane
would be too slow and mechanical stirring is therefore indispensable to ensure a fast access of the
compound to the membrane protein. Miniature stirrers were specially designed and conceived
with a proprietary structure to allow mixing of the solution in the 96 well format. Placing the
stirrers in the microtiter wells offers the additional advantage to reduce the volume of the well
and therefore to minimize the amount of solution required for the experiment.