Multichannel Systems HiClamp Manual - Manual (Page 36)

HiClamp Manual
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After clicking on "Done" the list window appears. Once defined you can change the active
definition with the "Edit" function.
Wash/Test
The "Wash/Test" icon is used to move the oocyte into the wash station where compounds can be
washed or a test solution can applied to test the oocyte response. Parameters corresponding to
this step can be accessed after a double click on the icon. These parameters include the amplifier
settings, the voltage step control and drug application for the four available channels. The wash /
test command is often used to determine the leak current of the cell, and its response to
a standard solution. Note that measurements will be carried out only if the data acquisition mode
is turned on.
The "If" Step
The “if” icon is designed to set up a detection mode that can be used for testing a given condition
(holding current or response) of the cell. Typical example of the use of the “if” are shown in the
concentration activation curve example. The “if” applies on data that have been captured in the
last data acquisition epoch. For example, by the wash / test in the acquisition mode or A/D.
Parameters governing the “if” action are set up in the associated window that is accessible by
a double click on this icon in the program sequence. On the first double click, the cursor changes
mode from the arrow to a circle to indicate the need for a user action. The user should then point
to the icon where the program sequence should step back when the “if” conditions are not met.