Multichannel Systems HiClamp Manual - Manual (Page 21)

HiClamp
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Cartoon of the automated oocyte transport from 96-well feeder plate to the oocyte basket:
The oocyte is in the selected well of the feeder plate (1). The suction tube is lowered into the
well (2). The oocyte is aspirated (3). The suction tube moves to the basket (4). The suction tube
is lowered directly above the basket (5). The suction tube emits the oocyte (6). The oocyte is
placed in the basket (7). The basket moves with the oocyte beneath the position of the TEVC
electrodes (8). The electrodes are lowered and the oocyte is impaled for recording (9).
Finally, the recording sequence starts when the oocyte is successfully impaled and an appropriate
resting potential is measured by both intracellular glass electrodes.