Multichannel Systems HiClamp Manual - Manual (Page 40)

HiClamp Manual
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5.7 Data Acquisition Icons
Execution of the icons described above allows the control of HiClamp movement and action.
Additional icons are, however, necessary to perform data acquisition. Three icons control the
data acquisition and can be used in the program sequence. At the end of each data acquisition
the window DataPlot is turned on to display the last recorded data period.
Important: The timing of the data acquisition must be verified and the program should be set
to loop back before data acquisition is completed.
Starting Data Acquisition
This icon is typically used to start the data acquisition and to move the basket into the different
compounds as shown in the concentration activation curve example. Execution of this icon
initiates the data acquisition and sets up the voltage command and the amplifier conditions.
The execution of the program sequence continues independently from data acquisition.
Recording in the washing station
This icon indicates that the data acquisition mode has been set in the parameters of the wash /
test icon. Setting up the data acquisition causes the recording of a data epoch, the parameters
of which are set by the user. Execution of the program will resume only when the recording time
is completed and the automate will remain in the wash chamber for the entire duration set in the
corresponding parameters.
: Recording in Plate
Similarly to the wash / test icon, this icon is displayed only when the data acquisition mode is
turned on in the corresponding plate parameters. Execution of this icon will turn on the data
acquisition and maintain the program sequence at this step until completion of the recording
time. This mode is particularly useful to determine voltage dependent characteristics in a given
solution, for example in the determination of the properties of voltage dependent channels.
Super Icons and loading stored sequences
As particular program sequence may often be used in multiple experiments it is simpler to define
them as Super Icons. Super Icons can be designed by the user or used from a standard icon pool.
To set a Super Icon that will be represented in the upper part of HiClamp program it is sufficient
to save the currently defined sequence. Upon saving the program sequence HiClamp issues a
request window for setting up or not the Super Icon that can be chosen amongst *.jpeg files. To
activate a Super Icon it is sufficient to click once on the Super Icon and the program automatically
loads the program sequence. Note that all the fields concerning compounds will automatically be
emptied.
An alternative way of loading a stored sequence consists in opening a sequence previously saved
in a given experiment. Activation of the command “File -> Open” allows to browse through
previously saved sequences and the user can chose any sequence generated by HiClamp. Upon
loading a stored sequence a dialog windows open requesting if the parameters of the sequence
must be kept. Hitting the button “Yes” confirms that all the values, including the compound
names and concentrations will be kept in the newly defined sequence. Alternatively, answering
“No” will load the sequence but empty the compound names and concentrations previously
defined. Note that names, values and concentrations must be filed in before using the sequence.