Multichannel Systems HiClamp Manual - Manual (Page 35)

TEVC Recording Protocol
29
5.6 Icons and the associated Effects
Oocyte Loading
This icon is used to select the oocytes that will be taken for the measurements. So it is normally
the first of a program sequence. Oocytes setting, such as the injected message, number of cells,
date of injection etc. are stored in the corresponding data file. Access to these parameters is
done by a double click on the "Oocyte Loading" icon and filling the appropriate parameters.
All parameters are automatically stored in the data sequence that is stored in the experiment
folder. Multiple message types (cDNAs or mRNAs) can be defined in a single loading plate
allowing determination of multiple protein functions.
Compound Plate
This icon is used to define the assembly of compounds in the compound plate and the sequence
of events that will be measured. Parameters such as incubation time, holding voltage, compound
localization, concentrations etc. are defined by the user by a double click on the icon.
If you check the "Acquisition" box, options for setting sampling and filter frequency and the
"Voltage Command" button appears. Double click on "Define Solutions" opens another window
in which you can define and attribute solutions or compounds to the respective compound well
(A1 to H12). If you want to define a sequence of concentration starting for example at A1,
you just have to type the concentrations separated by a space.