Multichannel Systems HiClamp Manual - Manual (Page 37)

TEVC Recording Protocol
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Following a double click on the desired icon the condition window is displayed.
“Go To Step” indicates the icon to which the “if” is returning when the condition are not met.
The “Leak” (expressed in μA) and current (expressed in μA) are used for the evaluation of the “if”.
Unless the cursor are set up (see below) values are determined on the 10 first data points for the
leak current and on the entire data epoch for the current. When the conditions are set a red line
is represented in the upper part of the sequence program to indicate how the sequence executes.
Equation is designed for future applications.
Loop with increment
The loop with increment causes the program sequence to loop back to the pointed icon and to
increment the position of the tested compound in the plate. For example, in the concentration
activation curve the loop with increment allows to measure successively the responses evoked
by a series of agonist concentrations. A double click on the loop icon changes the cursor from
arrow to circle, indicating that the user can now point to the icon where the loop should go back.
A double click on the desired icon turns on the corresponding window.
The current step indicates the icon position at which the loop was inserted. The loop to step
indicates the icon at which the loop is pointing and where the program sequence will step back
upon execution of the loop. The number of times indicates how many times the loop will be
executed. It is of good practice to always verify that this number corresponds to the desired
number of time of executions. This value can be manually changed to reflect the desired number
of time. When the button done is pushed this window automatically closes and a blue line is
represented under the program sequence to indicate how the loop will take place. The number
indicated in blue under the loop icon indicates the remaining number of passages. As error in
setting up the loop are easily made it is important to always double check before starting a given
sequence.